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Figure 7. Immunoblotting analysis of b-chitosan treatment on NF-kB signaling pathway in activated RAW264.7 cells (A and B) Immunoblotting analyses of the effects of b-chitosan on iNOS and CD86 in RAW264.7 cells with LPS stimulation (10 mg/mL). Quantifications of protein expressions are shown in (B). (C and D) Effect of b-chitosan on the expressions of TLR4, TNF-a and IL-1b in RAW264.7 under LPS stimulation. (E, F) The downstream signal molecules (p-JNK, p-p38 and <t>p-p65)</t> of NF-kB in RAW264.7 (stimulated by LPS) were regulated by b-chitosan. (G and H) Immunoblotting analysis (G) and quantification (H) of the effects of b-chitosan on TLR4, TNF-a and IL-1b in RAW264.7 cells with C12-sulfatide stimulation (10 mg/mL). Data are presented as mean G SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA.
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Figure 7. Immunoblotting analysis of b-chitosan treatment on NF-kB signaling pathway in activated RAW264.7 cells (A and B) Immunoblotting analyses of the effects of b-chitosan on iNOS and CD86 in RAW264.7 cells with LPS stimulation (10 mg/mL). Quantifications of protein expressions are shown in (B). (C and D) Effect of b-chitosan on the expressions of TLR4, TNF-a and IL-1b in RAW264.7 under LPS stimulation. (E, F) The downstream signal molecules (p-JNK, p-p38 and <t>p-p65)</t> of NF-kB in RAW264.7 (stimulated by LPS) were regulated by b-chitosan. (G and H) Immunoblotting analysis (G) and quantification (H) of the effects of b-chitosan on TLR4, TNF-a and IL-1b in RAW264.7 cells with C12-sulfatide stimulation (10 mg/mL). Data are presented as mean G SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA.
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Figure 7. Immunoblotting analysis of b-chitosan treatment on NF-kB signaling pathway in activated RAW264.7 cells (A and B) Immunoblotting analyses of the effects of b-chitosan on iNOS and CD86 in RAW264.7 cells with LPS stimulation (10 mg/mL). Quantifications of protein expressions are shown in (B). (C and D) Effect of b-chitosan on the expressions of TLR4, TNF-a and IL-1b in RAW264.7 under LPS stimulation. (E, F) The downstream signal molecules (p-JNK, p-p38 and p-p65) of NF-kB in RAW264.7 (stimulated by LPS) were regulated by b-chitosan. (G and H) Immunoblotting analysis (G) and quantification (H) of the effects of b-chitosan on TLR4, TNF-a and IL-1b in RAW264.7 cells with C12-sulfatide stimulation (10 mg/mL). Data are presented as mean G SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA.

Journal: iScience

Article Title: β-chitosan attenuates hepatic macrophage-driven inflammation and reverses aging-related cognitive impairment.

doi: 10.1016/j.isci.2024.110766

Figure Lengend Snippet: Figure 7. Immunoblotting analysis of b-chitosan treatment on NF-kB signaling pathway in activated RAW264.7 cells (A and B) Immunoblotting analyses of the effects of b-chitosan on iNOS and CD86 in RAW264.7 cells with LPS stimulation (10 mg/mL). Quantifications of protein expressions are shown in (B). (C and D) Effect of b-chitosan on the expressions of TLR4, TNF-a and IL-1b in RAW264.7 under LPS stimulation. (E, F) The downstream signal molecules (p-JNK, p-p38 and p-p65) of NF-kB in RAW264.7 (stimulated by LPS) were regulated by b-chitosan. (G and H) Immunoblotting analysis (G) and quantification (H) of the effects of b-chitosan on TLR4, TNF-a and IL-1b in RAW264.7 cells with C12-sulfatide stimulation (10 mg/mL). Data are presented as mean G SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA.

Article Snippet: Antibodies Rabbit monoclonal anti-VCAM1 Abcam Cat#ab134047; RRID: AB_2721053 Rabbit polyclonal anti-CD68 ProteinTech Cat#28058-1-AP; RRID: AB_2881049 Rabbit polyclonal anti-IBA1 ProteinTech Cat#10904-1-AP; RRID: AB_2224377 Mouse monoclonal anti-IL-1b Santa Cruz Biotechnology Cat#SC-52012 Rabbit polyclonal anti-TNF-a Affinity Cat#AF7014 Rabbit polyclonal anti-NLRP3 Affinity Cat#DF7438; RRID: AB_2839376 Rabbit polyclonal anti-MMP9 Abmart Cat#TA0220 Rabbit polyclonal anti-IL-6 Abmart Cat#TD6087 Rabbit polyclonal anti-iNOS Affinity Cat#AF0199; RRID: AB_2833391 Rabbit polyclonal anti-CD86 Affinity Cat#DF6332; RRID: AB_2838296 Rabbit polyclonal anti-TLR4 ProteinTech Cat#19811-1-AP; RRID: AB_10638446 Rabbit polyclonal anti-JNK ProteinTech Cat#24164-1-AP; RRID: AB_2879443 Rabbit polyclonal anti-p38 ProteinTech Cat#14064-1-AP; RRID: AB_2878007 Rabbit polyclonal anti-p65 ProteinTech Cat#10745-1-AP; RRID: AB_2178878 Rabbit polyclonal anti-p-JNK Affinity Cat#AF3318 Rabbit polyclonal anti-p-p38 Affinity Cat#AF4001 Rabbit polyclonal anti-p-p65 Affinity Cat#AF2006 Rabbit polyclonal anti-IL-1b Affinity Cat#AF5103 Rabbit polyclonal anti-IL-1b ProteinTech Cat#16806-1-AP Rabbit polyclonal anti-b-tubulin Affinity Cat#AF7018 Rabbit polyclonal anti-GAPDH Affinity Cat#AF7021; RRID: AB_2839421 Rabbit polyclonal anti-b-actin Affinity Cat#AF7011 Mouse monoclonal anti-F4/80 Santa Cruz Biotechnology Cat#SC-377009; RRID: AB_2927461 Mouse monoclonal anti-CD68 Novus Biologicals Cat#NB100-683 Mouse monoclonal anti-TNF-a ProteinTech Cat#60291-1-Ig

Techniques: Western Blot